Biochemical analysis of ascorbic acid assay in human saliva throughout menstrual cycle

The recent finding shows that the ascorbate involves in gamete protection and tissue remodelling. The damage of gametes by free radicals prevented by ascorbic acid and on the whole the preliminary exploration of the ascorbic acid involves directly or indirectly in fertilization or its interrelated actions. Twenty-three females, aged between 18 and 40 years, were recruited for the study. Subjects were not using oral contraceptive pills and had regular menstrual cycles. Ascorbic acid level was measured daily in the first morning specimen of saliva in five ovulating and non-ovulating females who were receiving 500 mg ascorbic acid each morning at breakfast throughout the menstrual cycle. Ascorbic acid level showed massive variations associated with an alteration in basal body temperature and trans-vaginal sonograph (≤ 4 mm size) during the 14 day of the ovulation phase. There was a slight fluctuation during the subsequent fall in ascorbic acid secretion on day 16, and the flow of ascorbic acid diminished further until the 18 and 19 day. The pattern of ascorbic acid secretion closely resembles the flow of luteinizing hormone (LH) at the time of human ovulation. These results provide direct evidence on which theories for the women ovulation cycle can be assessed.